preparative sds-page electroelution for rapid purification of alkyl hydroperoxide reductase from helicobacter pylori

background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. there­fore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyranoside. the isolation and purification of  ahpc protein were attempted by various techniques including ammonium sulfate precipitation, dialysis, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and electroelution. results: a simple method was used for protein purification ahpc protein. one-dimensional preparative gel electrophoresis allows a single and short purification step; the high resolution capacity of this technique leads to a high level of purity of the protein. moreover, it avoids contamination by other non-specific proteins which often appear during protein purification by column chromatography. conclusion: the present method is simple, rapid and makes it possible to preparate ahpc from h. pylori